You will hear the following terms used during the interview. I've included some descriptions here.
- PCR - The Polymerase Chain Reaction is a technique for amplifying DNA sequences using a thermostable DNA polymerase enzyme and a cycling reaction temperature profile of heating and cooling. Dedicated instruments typically provide the temperature cycling mechanism.
- LAMP reaction – The Loop-mediated isothermal AMPlification reaction is also used to amplify DNA sequences. Unlike PCR, LAMP reactions occur at a single temperature (typically ~60 oC – 65 oC). Therefore, they do not require dedicated instruments to provide thermal cycling but can be conducted in simple water baths or heating blocks. LAMP also uses a DNA polymerase enzyme, although a different one from PCR. LAMP typically produces higher DNA yields than PCR
- CRISPR – CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats, a family of specific DNA sequences in prokaryotes. Enzymes, such as 'Cas9' can modify DNA at these CRISPR sequences, editing in or out new DNA sequences. Although this technique is known correctly as CRISPR-Cas9, the discovery and development of a range of CRISPR-associated enzymes has led to the term 'CRISPR' being used as a generic term for this suite of gene editing technologies.
- PDMS – PolyDiMethylSiloxane is the most widely used silicon-based organic polymer. It is optically clear, usually considered inert, non-toxic, and non-flammable. It is used in a wide range of medical, food, and industrial processes.
As we mentioned during the podcast, Vittorio has several videos and public announcements explaining his research areas. These include:
LAMP SARS-Cov-2 assay in a Nespresso capsule
Microfluidics
3D printing dichroic nanocomposites (Lycurgus cup)
Standard 3D printers for microfabrication
And some Arduino
Vittorio's publications are all at
Vittorio (and his research) is very present on social media, you can find him on
LinkedIn at https://www.linkedin.com/in/vittorio-saggiomo-42920617b/,
On Twitter, search @V_Saggiomo
Vittorio's publications are all at https://scholar.google.nl/citations?hl=en&user=AlppnJ4AAAAJ
Our theme music is "Wholesome" by Kevin MacLeod (https://incompetech.com)
Music from https://filmmusic.io
License: CC BY (http://creativecommons.org/licenses/by/4.0/)
Connect with me (Paul) at https://www.linkedin.com/in/paulorange/
H.E.L. group can be found at
www.helgroup.com
online,
on LinkedIn at
https://www.linkedin.com/company/hel-group/
on Twitter we're @hel_group,
https://twitter.com/hel_group
or search for us on Facebook
